Nanohybrid-based Detection and Separation

Participants focus on molecular interactions on or within nanohybrid-based devices, built upon the discoveries within the first two research clusters to develop sensing and separation devices able to address challenges of importance in providing clean water, improving health and wellness, and reducing environmental hazards and enhancing national security.

This cluster combines the most vibrant and emerging areas of research in CNFM. Selected research focii of participants are highlighted below.

Cluster 3 Research Foci:

Imaging Instrumentation and Methodology.

Participants currently design and construct imaging instrumentation capable of monitoring molecular interactions on 3-D nanostructures. Model approaches seek to quantify characteristic properties such as target presence and spatial distributions across 3-D nanostructures.

Keywords: Imaging Polarimetry and Ellipsometry; Generalized Ellipsometry; Surface-enhanced microscopy; Birefringence Imaging Microscopy; Confocal Microscopy; AFM.

Birefringence Imaging Chromatography (Two-dimensional Chromatography).

Participants currently exploit new instrumentation capable for simultaneous separation as well as imaging of molecular interactions on 3-D nanostructures. This research may lead to new approaches in chromatography.

Keywords: Chromatography; Affinity-based chromatography; Electro-chromatography; Two-dimensional chromatography; Environmental transport; Fate and transport of contaminants.

Cell-instructive surfaces.

Participants investigate the potential of 3-D nanohybrids for their potential to influence and monitor cell behavior.

Keywords: Surface-mediated DNA delivery; Cell-instructive surfaces; Biofunctional surfaces.


Cluster 3 Recent Research Highlights:

Analysis of Biomolecular Interactions using Affinity Microcolumns: A Review.
Reactive Arrays of Colorimetric Sensors for Metabolite and Steroid Identification.
Alpha-synuclein misfolding assessed with single molecule AFM force spectroscopy: effect of pathogenic mutations.
Nanoimaging for protein misfolding diseases.
Affinity Monolith Chromatography: A Review of Principles and Recent Analytical Applications.
Fabrication of Electrochemical DNA Sensors on Gold-modified Recessed Platinum Nanoelectrodes.
Nanoimaging methods for biomedicine.

Cluster 3 Researchers:

Mathias Schubert - Generalized Ellipsometry; Imaging; Anisotropic materials. Tino Hofmann - Optical instrumentation design; THz Ellipsometry. Yuri Lyubchenko - Imaging of biopolymer nanoassemblies; AFM force spectroscopy. Rebecca Lai -  Electrochemical sensors. Angela Pannier -  Confocal and Fluorescence Microscopy. David Hage - Chromatography and electro-chromatography.  Yusong Li - Engineered nanoparticles. Shannon Bartelt-Hunt - Prion folding and propagation.